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The increases in their expression can impact AR in a variety of manners, including stabilization of AR mRNA through RNA-RNA hybridization (ARLNC1 [117]), absorption of miRNAs (plncRNA-1 [118]), facilitation of mRNA splicing (PRKAG2-AS1, HOXC-As1 [119]), interference with epigenetic control over AR target genes (SOCS2-AS1 [120]), preventing AR degradation (HOTAIR [121]), regulating its binding to chromatin (PCAT1 [122]) or binding to AR and thereby enhancing its transactivation (PCAT8, PCGEM1 [123])

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A more evidence-based way to evaluate whether something is helping is to track measurable outcomes with your clinician (for example, symptoms tied to confirmed deficiency, or objective metrics like body weight, waist measurements, and lab work when appropriate)

PCR conditions were 94 C for 30 s